Characterisation of the novel SWR1 complex components.

13 proteins with a positive or unknown NES were identified by MS analysis in at least three of the four co-IP experiments. The initial co-IP was performed with RuvB2 (Tb927.4.2000, the reciprocal co-IPs with the proteins Tb927.10.11690, Tb927.11.5830 and Tb927.7.4040 were performed to confirm the RuvB2 co-IP data). The “Annotation” column indicates the curated annotation that was found for the corresponding accession number in the TriTryp database. The”identified domains” column displays the domains that were found by BLAST search using the NCBI database. The Phyre2 modelling column indicates proteins that were identified by homology modelling. Coverage (Cov.) indicates the coverage in percent between query and template. The confidence (Conf.) represents the relative probability in percent (from 0 to 100) that the match between query and template is a true homology. The nuclear enrichment score (NES; (52)) indicates the probability of a nuclear localisation based on cell fractionation combined with quantitative MS analysis. The column "TrypTag localisation” indicates the cellular localisation of N- and C-terminally tagged proteins. The last column shows in which of the four co-IPs the protein could be identified.本研究通过质谱（mass spectrometry, MS）分析鉴定得到13种蛋白质，此类蛋白质在4次免疫共沉淀（co-immunoprecipitation, co-IP）实验中至少有3次被检出，且其核输出信号（nuclear export signal, NES）呈阳性或未注释状态。首次免疫共沉淀实验以RuvB2（Tb927.4.2000）作为诱饵蛋白完成；为验证RuvB2的免疫共沉淀结果，分别针对蛋白质Tb927.10.11690、Tb927.11.5830及Tb927.7.4040开展了反向免疫共沉淀实验。"注释"列代表在TriTryp数据库中针对对应登录号所整理的注释信息；"鉴定结构域"列展示了通过NCBI数据库进行BLAST比对所发现的蛋白质结构域。"Phyre2建模"列标注了通过同源建模技术鉴定得到的蛋白质；覆盖度（Cov.）表示查询序列与模板序列之间的百分比匹配覆盖度；置信度（Conf.）代表查询序列与模板序列匹配为真实同源关系的相对概率百分比，取值范围为0至100。核富集评分（NES；参考文献52）反映了基于细胞分级分离结合定量质谱分析所得到的蛋白质核定位概率；"TrypTag定位"列标注了N端及C端标签融合蛋白质的细胞定位情况；最后一列则展示了该蛋白质可在4次免疫共沉淀实验中的哪几次被成功鉴定。