domino2: data for a reproducible example

This repository hosts example data for reproducible analysis of intra- and intercellular signaling in single cell RNA sequencing (scRNAseq) data based on transcription factor (TF) activation. We demonstrate analysis using domino2 on the 10X Genomics Peripheral Blood Mononuclear Cells (PBMC) data set of 2,700 cells PBMC3K. scRNA-seq data is preprocessed following the Satija Lab's Guided Clustering Tutorial. Quantification of TF activation is conducted using pySCENIC. For more details on how this analysis is conducted, please refer to the vignettes in the domino2 package.本代码仓库提供基于转录因子（transcription factor，TF）激活分析的单细胞RNA测序（single cell RNA sequencing，scRNAseq）数据中细胞内与细胞间信号传导的可复现分析示例数据。本研究针对10X基因组学（10X Genomics）发布的包含2700个细胞的外周血单个核细胞（Peripheral Blood Mononuclear Cells，PBMC）数据集PBMC3K，使用domino2工具完成信号传导分析演示。本数据集的scRNA-seq数据已按照Satija实验室的《引导性聚类教程》完成预处理，转录因子激活的量化分析依托pySCENIC工具完成。如需了解该分析的更多实施细节，请参阅domino2软件包中的vignettes文档。