Schistosomula exposed to HDAC inhibitor (Trichostatin A) [24hrs]. Schistosoma mansoni

HDACs inhibitors induces mortality in the parasite Schistosoma mansoni (schistosomula and adult worms), and became an interesting drug class for the development of new drugs to treat schistosomiasis. In order to understand the effect of histone hyperacetylation on the parasite, we tested the effect of the HDAC inhibitor Trichostatin A on schistosomula gene expression. Overall design: Newly transformed schistosomula were exposed to 1 µM Trichostatin A or vehicle solution (ethanol) and kept in culture for 24 h. Three independent biological replicates were assayed for each condition.

组蛋白去乙酰化酶（HDAC）抑制剂可导致曼氏血吸虫（Schistosoma mansoni）的童虫与成虫死亡，因此成为抗血吸虫病（schistosomiasis）新药研发的极具潜力的药物候选类别。为探明组蛋白高度乙酰化对该寄生虫的生物学效应，本研究检测了HDAC抑制剂曲古抑菌素A（Trichostatin A）对曼氏血吸虫童虫基因表达的调控作用。实验设计概述：将刚转化的曼氏血吸虫童虫暴露于1 µM曲古抑菌素A或溶剂对照（乙醇）中并体外培养24小时，每组设置3次独立生物学重复并开展检测。