Metabolic programming drives proximal tubule differentiation and kidney dysfunction

Renal disease development is poorly understood, mostly because the kidney is made up of more than 20 different cell types, each of them with specific function and complex interaction. Here, we used single cell RNA sequencing to resolve cell-type specific and cell fraction changes Single-cell RNA-seq of engineered human kidney organoids肾脏疾病的发生发展机制迄今尚未被充分阐明，这主要是由于肾脏由20余种不同的细胞类型构成，每类细胞均具备独特的功能且彼此间存在复杂的相互作用。本研究对工程化人类肾脏类器官进行单细胞RNA测序（single cell RNA sequencing），以此解析细胞类型特异性特征及细胞组分比例的变化