Homo sapiens Raw sequence reads

The human MDA MB 231 breast cancer and MDA MB 435 melanoma cell lines were selected for isolates able to pass through narrow 3 micron pores in Transwell tissue culture inserts. In addition, MDA MB 231 breast cancer cells were selected for a population of small sized cells in parallel by flow cytometric sorting. RNA sequencing of the three populations (parental, selected, flow sorted) of MDA MB 231 breast cancer cells, and two populations (parental, selected) of MDA MB 435 melanoma cells, was performed.本研究选取人源MDA MB 231乳腺癌细胞系与MDA MB 435黑色素瘤细胞系，用于筛选可穿透Transwell组织培养插入件中3微米窄孔的细胞群体。除此之外，研究同时通过流式细胞分选（flow cytometric sorting）对MDA MB 231乳腺癌细胞进行分选，富集得到小尺寸细胞亚群。研究人员随后对MDA MB 231乳腺癌细胞的三个群体（亲本细胞、Transwell筛选组、流式分选组），以及MDA MB 435黑色素瘤细胞的两个群体（亲本细胞、Transwell筛选组）开展了RNA测序（RNA sequencing）。