Supplemental Material for Warsinger-Pepe, Li, and Yamashita, 2020

Figure S1 shows representative images of diploid larval tissues revealing that heterochromatin formation aids in Y rDNA dominance in males. Figures S2 shows quantification of nucleolar dominance in polyploid tissues in wild type and mutant males. Figure S3 shows RNA in situ controls revealing that distinct ITS probes can differentially identify X rDNA variants. Figure S4 shows hybrid dysgenesis data for female nucleolar dominance crosses and that female nucleolar dominance is not influenced by parental origin. Figure S5 shows DNA FISH and IF images of the cytological characterization of the Xbb-YS chromosome. Reagents Table lists all relevant reagents used in this manuscript. Table S1 compiles the raw data of nucleolar dominance scoring for each relevant figure, organized by tab.图S1展示了二倍体幼虫组织的代表性图像，揭示异染色质形成可促进雄性个体的Y核糖体DNA（rDNA）显性现象。 图S2展示了野生型与突变型雄性多倍体组织中核仁显性的定量分析结果。 图S3展示了RNA原位对照实验，证实不同的内转录间隔区（ITS）探针可差异化识别X染色体rDNA变异亚型。 图S4展示了雌性核仁显性相关杂交的杂种劣生实验数据，表明雌性核仁显性不受亲本来源的影响。 图S5展示了Xbb-YS染色体细胞学鉴定的DNA荧光原位杂交（DNA FISH）与免疫荧光（IF）图像。 试剂表列出了本论文中使用的全部相关实验试剂。 表S1汇总了各相关图对应的核仁显性评分原始数据，并按工作表进行分类整理。