Targeted exome intervals for snowshoe hare

A .bed file containing the coordinates of the targeted exome regions for snowshoe hares based on the European rabbit (oryCun2.0) genome. The capture was originally designed by Jones et al. 2018 (linked in references).<br><br>We targeted all protein-coding and untranslated regions (UTRs) annotated in the European rabbit reference genome and novel UTRs identified from published molting snowshoe hare skin transcriptomes (total of207,691 regions, 55.29-Mb) (Ferreira et al. 2017, linked in references). Custom probes were generated by Roche (NimbleGen SeqCapEZ Developer) using a relaxed probe design. The final capture probe set covered 97.4% of originally targeted bases (&gt;99% of protein-coding genes) and included 213,164 probes covering 61.73-Mb (~25-Mb protein-coding, ~28-Mb UTR, ~9-Mb intron/intergenic).<br>本数据集包含基于欧洲兔（oryCun2.0）参考基因组的雪兔目标外显子组区域坐标的BED格式文件。该靶向捕获探针组最初由Jones等人于2018年设计（参考文献中附链接）。 本研究靶向了欧洲兔参考基因组中注释的所有蛋白编码区与非翻译区（Untranslated Regions, UTRs），以及从已发表的换毛期雪兔皮肤转录组中鉴定得到的新型UTR，总计207691个区域，总长度为55.29 Mb（Ferreira等人，2017年，参考文献中附链接）。 定制探针由罗氏（Roche）旗下的NimbleGen SeqCapEZ开发平台采用宽松探针设计策略制备。 最终的靶向捕获探针组覆盖了97.4%的初始靶向碱基（覆盖99%以上的蛋白编码基因），共包含213164条探针，总覆盖长度为61.73 Mb，其中蛋白编码区约25 Mb、UTR区约28 Mb、内含子/基因间区约9 Mb。