Depot dependent effects of dexamethasone on gene expression in human omental and abdominal subcutaneous adipose tissues from obese women.

We used microarrays to identify transcripts regulated by dexamethasone in omental (Om) and abdominal subcutaneous (Abdsc) adipose tissues of severely obese females obtained during elective surgeries. Minced tissue was treated with Dexamethasone in organ culture for 7 days as previously described (Lee et al, Am J Physiol Endocrinol Metab. 2011 Mar;300(3):E571-80). 3 samples of Abdsc cultures and Om from the same individual was cultured in the presence of 0.7 nM insulin plus 0,1,10 or 1000 nM Dexamethasone. Adipose tissue from subject A was similtaneously cultured with 200 nM cortisol. Sample A is from one subject, Sample B and C are pooled from two different subjects each.

本研究采用基因芯片（microarrays）技术，对择期手术获取的重度肥胖女性的网膜（Om）及腹部皮下（Abdsc）脂肪组织中受地塞米松（dexamethasone）调控的转录本进行筛选。将切碎的脂肪组织置于器官培养体系中，以地塞米松处理7天，实验流程参照既往发表的研究方法（Lee等，《美国生理学杂志·内分泌与代谢》，2011年3月；300(3):E571-80）。取同一受试者的3份腹部皮下脂肪组织培养样本及网膜脂肪组织，在含有0.7 nM胰岛素以及0、1、10或1000 nM地塞米松的培养体系中进行培养。受试者A的脂肪组织同时以200 nM皮质醇（cortisol）进行培养。样本A来源于1名受试者，样本B与样本C则分别由2名不同受试者的脂肪组织混合制备。