CHO-S transcriptome in batch culture

Transcriptomic data from CHO-S cells were gathered from multiple time points during batch culture to provide information about gene presence/absence for generation of a CHO-S cell line specific model using the GIMME algorithm. CHO-S cells were grown in batch culture (n=4), samples were taken every 12 hours between t=72 and t=180h for sequencing.本研究采集了分批培养（batch culture）过程中多个时间点的CHO-S细胞转录组数据（transcriptomic data），以获取基因存在与缺失的相关信息，用于基于GIMME算法（GIMME algorithm）构建CHO-S细胞系特异性模型。本次实验中CHO-S细胞采用分批培养体系，设置4个生物学重复（n=4），在t=72h至t=180h的培养区间内每12小时采集一次样本用于测序。