Mapping dynamic Tle3 distribution during CD8 T cell responses

The goal of this study is to determine if central memory (Tcm) and effector memory (Tem) CD8 T cells can be reprogrammed to change their fate. We demonstrate that genetic ablation of Tle3 can promote generation of Tcm cells at the expense of Tem cells, and this can occur during the effector phase of the immune response. Overall design: Genome-wide mapping of transcriptomic, chromatin accessibility, and Tle3 binding profiles of WT naïve and effector cells, WT and Tle3-deficient central/effector memory CD8+ T cells.本研究旨在探究中枢记忆性（Tcm）与效应记忆性（Tem）CD8阳性T细胞是否可被重编程以改变其细胞命运。我们证实，Tle3基因的遗传敲除可促进中枢记忆性CD8 T细胞的生成，同时以效应记忆性CD8 T细胞的生成为代价，且该过程可发生于免疫应答的效应阶段。实验整体设计：对野生型（wild type, WT）未致敏细胞与效应细胞、野生型及Tle3缺陷型中枢/效应记忆性CD8阳性T细胞的转录组、染色质可及性及Tle3结合谱进行全基因组范围内的图谱绘制。