Ribosomal footprinting of CN34-Parental and CN34-LM1a

We used Illlumina Artseq Ribo Profile to perform ribosome footprinting on the above cell lines Overall design: We followed the manufacturer's protocol: Cells were treated with cycloheximide (0.1 mg/ml) to inhibit translation; total RNA(tt) and ribosome-protected RNA fragments (RPF) were isolated and library preped for sequencing simultaneously.我们采用Illumina Artseq Ribo Profile对上述细胞株开展核糖体足迹实验（ribosome footprinting）。实验设计概述：我们严格遵循厂商提供的实验方案：先用环己酰亚胺（cycloheximide，0.1 mg/ml）处理细胞以抑制翻译进程；随后同步分离总RNA（total RNA）与核糖体保护RNA片段（ribosome-protected RNA fragments, RPF），并同步为两类样本完成测序文库制备。