Results of RNA-seq of ribosome fractions from control and TSC2 KO Day37 organoids

mTOR pathway is a major regulator of translation in eukaryotic cells. Here we investigate the impact of mTOR overactivation through TSC2 KO on the translatome of brain organoid tissue. Overall design: Day37 organoids were dissociated and the lysate was used for polysome profiling. Monosome, low-polysome and high-polysome fractions were separately collected and the RNA associated with these fractions was isolated and used for RNA-seq

哺乳动物雷帕霉素靶蛋白（mTOR）通路是真核细胞翻译过程的主要调控因子。本研究探讨了经TSC2敲除（TSC2 KO）介导的mTOR过度活化对脑类器官组织翻译组（translatome）的影响。
总体实验设计：将培养至第37天的脑类器官解离，其裂解液用于多聚核糖体谱分析（polysome profiling）；分别收集单核糖体、低多聚核糖体与高多聚核糖体组分，分离与这些组分结合的RNA并进行RNA测序（RNA-seq）。