Cellular barcodes in human fibroblasts and human induced pluripotent stem cells extracted from bulk genomic DNA and linked with cellular barcodes extracted from single-cell copy DNA

argeted sequencing of barcodes from genomic DNA harvested from barcoded human fibroblast-like cells (hiF-T cells) and human induced pluripotent stem cells (hiPS cells) derived from hiF-T cells. hiF-T cells were transduced with barcode libraries consisting of semi-random 100-mers in the 3' UTR of GFP and permitted to undergo several cell divisions before being split and seeded for parallel reprogrammings. Both barcoded hiF-T cells and hiPS cells were harvested for isolation of genomic DNA before we performed targeted amplification of the barcode vector for sequencing. See Jain et al. citation for additional details.

本数据集源自对两类细胞基因组DNA中整合的条形码序列的靶向测序：一类为带条形码的人成纤维细胞样细胞（hiF-T cells），另一类是由hiF-T细胞诱导获得的人诱导多能干细胞（hiPS cells）。研究人员首先将携带半随机100聚体条形码文库的载体转导至hiF-T细胞，该文库整合于绿色荧光蛋白（Green Fluorescent Protein, GFP）的3'非翻译区（3' UTR）；待细胞完成数次分裂后，将其传代接种并开展平行重编程实验。随后收集全部带条形码的hiF-T细胞与hiPS细胞以分离基因组DNA，再对条形码载体进行靶向扩增并完成测序。更多实验细节可参考Jain等人的相关文献。