Expression data from superior cervical ganglion cultured cells and whole tissue

A transcriptomic expression comparison was done among superior cervical ganglion (SCG) cells cultured on 2D substrates, 3D porous polystyrene scaffolds, and in freshly dissected tissue (in vivo surrogate), with the goal of assessing the feasibility of establishing the meaning of 3D and associated physiological relevance at the molecular level SCG cells were cultured on 2D surfaces and in 3D scaffolds. Chemical cues are controlled by coating. Only spacial properties of the culture systems were compared. Cells from freshly dissected tissue were used as in vivo surrogates for positive control of 3D cells.本研究针对培养于二维底物（2D substrates）、三维多孔聚苯乙烯支架（3D porous polystyrene scaffolds）的颈上神经节（superior cervical ganglion, SCG）细胞，以及新鲜解剖组织（体内替代样本）开展转录组表达对比分析，旨在从分子层面评估明确三维培养体系的应用价值及其相关生理相关性的可行性。实验中，SCG细胞分别培养于二维表面与三维支架中，培养体系的化学信号通过表面包被操作进行严格控制，本次对比仅针对不同培养系统的空间特性展开。新鲜解剖获取的组织细胞被用作三维培养细胞的阳性对照体内替代样本。