Adult neurogenesis improves spatial information encoding in the mouse hippocampus - hM4Di Silencing
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In vivo two-photon imaging dataset for Frechou et al. "Adult neurogenesis improves spatial information encoding in the mouse hippocampus" This dataset includes data from CNO-induced chemogenetic silencing experiments in hM4Di+ and hM4Di-(control) mice. There is another Zenodo dataset with the remaining hM4Di-(control) mice here. The folder for each mouse includes baseline (pre) and post-CNO (cno) recordings. For each recording we included raw imaging data consisting of: Individual frames (.tif files) from 3 consecutive 3 min Ca2+ imaging movies (which were concatenated for analysis) Microscope settings metadata (Experiment.xml) Mouse location data (Episode001.h5 in SyncData folder) containing rotary encoder and RFID data Some analyzed data is also included: Suite2p analysis data (Suite2p folder) fluorescence.npy contains raw fluorescence data (the F output of Suite2p data extraction). Rows are individual cells and columns are frames (i.e. timepoints) acquired at 15.253 Hz. positions.npy contains the position of the mouse on the treadmill belt indexed from 0 to 100. Both NumPy(.npy) files are the output of the Preprocessing.py code, part of the analysis pipeline used for data analysis in the original publication, which can be found at https://github.com/GoncalvesLab/Frechou-et-al-Neurogenesis Refer to the original publication for additional information. The sex of individual mice is as follows: Group Mouse # Sex Group Mouse # Sex hM4Di+ M1 M hM4Di- M1 M M2 M M2 M M3 F M3 M M4 F M5 M M6 M M7 M M8 F M9 F
适配Frechou等人《成年神经发生改善小鼠海马体空间信息编码》研究的在体双光子成像（in vivo two-photon imaging）数据集。 本数据集包含hM4Di+组与hM4Di-（对照组）小鼠经氯氮平-N-氧化物（CNO）诱导的化学遗传学沉默（chemogenetic silencing）实验数据。另有一份Zenodo数据集收录了剩余的hM4Di-（对照组）小鼠实验数据。 每只小鼠对应的文件夹中包含基线（给药前，pre）与CNO给药后（cno）的记录数据。 每条记录包含的原始成像数据如下： 1. 三段连续3分钟的钙成像（Ca²+ imaging）电影的单帧图像（.tif文件），分析时将其拼接为完整序列； 2. 显微镜设置元数据（Experiment.xml文件）； 3. 同步数据（SyncData）文件夹内的Episode001.h5文件，其中存储了小鼠位置数据，包含旋转编码器与射频识别（RFID）相关信息。 本数据集同时包含部分已处理的分析数据： 1. Suite2p分析数据（Suite2p文件夹）； 2. fluorescence.npy：存储原始荧光数据（即Suite2p数据提取流程输出的F结果）。数据行对应单个细胞，列对应采集帧（即时间点），采集帧率为15.253 Hz； 3. positions.npy：存储跑步机跑带上小鼠的位置数据，索引范围为0至100。 上述两份NumPy（.npy）格式文件均由Preprocessing.py代码生成，该代码是原论文数据分析流程的组成部分，相关代码可通过链接https://github.com/GoncalvesLab/Frechou-et-al-Neurogenesis 获取。 更多详细信息请参阅原研究论文。各小鼠的性别信息如下： | 分组 | 小鼠编号 | 性别 | 分组 | 小鼠编号 | 性别 | |----------|----------|------|------------|----------|------| | hM4Di+ | M1 | 雄 | hM4Di- | M1 | 雄 | | | M2 | 雄 | | M2 | 雄 | | | M3 | 雌 | | M3 | 雄 | | | M4 | 雌 | | | | | | M5 | 雄 | | | | | | M6 | 雄 | | | | | | M7 | 雄 | | | | | | M8 | 雌 | | | | | | M9 | 雌 | | | |
