Conjoint analysis of transcriptional and protein changes in heterosis of hybrid yellow catfish Huangyou-1 uncovers key roles for miRNAs [RNA-Seq]

In this study, we used next-generation sequencing technologies and tandem mass tags to characterize mRNA-seq, miRNA-seq and proteomic of Pelteobagrus fulvidraco, P. vachelli and hybrid yellow catfish Huangyou-1 (P. fulvidraco female×P. vachelli male) livers and in doing so, offer deeper insight into the transcriptional and protein changes in heterosis uncovers key roles for miRNAs. Overall design: RNA-sequencing analysis was performed on an Illumina Hiseq 2500 (LC Sciences, USA) following the vendor's recommended protocol

本研究采用下一代测序技术与串联质量标签（tandem mass tags, TMT）技术，对黄颡鱼（Pelteobagrus fulvidraco）、瓦氏黄颡鱼（Pelteobagrus vachelli）及其杂交品种黄优1号（P. fulvidraco ♀ × P. vachelli ♂）的肝脏组织开展mRNA测序（mRNA-seq）、微小RNA测序（miRNA-seq）以及蛋白质组学分析；借此深入解析杂种优势相关的转录与蛋白水平变化，并揭示微小RNA在此过程中的关键调控作用。实验设计概述：参照厂商推荐的实验流程，使用Illumina HiSeq 2500测序平台（美国LC Sciences公司）完成RNA测序分析。