Targeted modulation of chicken genes in vitro using CRISPRa and CRISPRi toolkit

To investigate the effectiveness of CRISPR based gene modulation, IRF7 was repressed in dCas9-KRAB DF1 cell line or activated in dCas9-VPR DF1 cell line followed by whole transcriptome analysis a. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-KRAB DF1 cells for assessing the endogenous targeted gene knockdown b. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-VPR DF1 cells for assessing the endogenous targeted gene activation为探究基于成簇规律间隔短回文重复序列（CRISPR）的基因调控效能，本研究在dCas9-KRAB DF1细胞系中抑制IRF7基因表达，或在dCas9-VPR DF1细胞系中激活IRF7基因表达，随后开展全转录组分析a。对转染非靶向向导RNA（gRNAs）的dCas9-KRAB DF1细胞（mock非靶向对照组）与转染靶向IRF7的向导RNA的细胞组进行差异基因表达分析，以评估内源靶基因的敲降效果b。对转染非靶向向导RNA（gRNAs）的dCas9-VPR DF1细胞（mock非靶向对照组）与转染靶向IRF7的向导RNA的细胞组进行差异基因表达分析，以评估内源靶基因的激活效果。