CFTR dependent response. Homo sapiens

Wild-type and dF508 CFTR mutant human bronchial epithelial cells were uninfected or infected for 3 hours at an MOI of 30-50 with P.aeruginosa strain PAO1. Each cell line was tested in 4 replicates. Each replicate consists of 4 data sets: 2 sets of duplicate spots on 2 arrays. The two arrays in each replicate were dye-swapped pairs where the opposite dye was used for each sample in the second array. I have called replicates "Rep1, Rep2..." etc. I have indicated duplicates from the same slide as eg."Slide1A and Slide1B" I have refered to the two arrays used in each replicate as "Slide1" and "Slide 2". Keywords: other野生型及ΔF508型囊性纤维化跨膜传导调节因子（CFTR）突变体人支气管上皮细胞，分别以感染复数（MOI，Multiplicity of Infection）30~50的铜绿假单胞菌（Pseudomonas aeruginosa, P.aeruginosa）菌株PAO1进行未感染处理，或感染3小时。每个细胞系设置4次重复实验。每次重复实验包含4组数据集：2张芯片上各设有2组重复点样。每次重复中的两张芯片为染料互换配对组，即第二张芯片中每个样本所使用的荧光标记染料与第一张芯片相反。本研究中将重复实验命名为“Rep1、Rep2……”等；将同一张玻片上的重复样本标注为例如“Slide1A与Slide1B”；将每次重复实验所用的两张芯片分别称为“Slide1”与“Slide2”。关键词：其他