DNA markers used for fine mapping spring1 on chromosome 7.

DNA markers from the MtCO region of chromosome 7 were analysed for linkage to spring1 in the mapping crosses (see Text). The interval containing spring1 is flanked by Medtr7g084170.1 and Medtr7g085190.1 shown in bold; each marker is separated from spring1 by one recombination event.aDNA marker taken from the University of Minnesota (UMN) Integrated Genetic Map of Medicago truncatulahttp://www.medicago.org/genome/map.php.bDNA marker from Pierre et al (2010).cSize of PCR fragment predicted from Mt3.5 Genome assembly from http://medicagohapmap.org/.dNumbers of recombinants detected by the marker in the late or early flowering classes from the F2 of the “spring1 x Jester” cross, or from the Test cross (see Table 1 and Text). “Test Cross” is (♂(♂spring1 x ♀Jester) x ♀Jester). NT is not tested. Indel is insertion/deletion; SSR is Simple Sequence Repeat. The column entitled Mt3.5 Genome assembly gives the position of the marker on the pseudomolecule from Mt3.5 Genome assembly from http://medicagohapmap.org/after a Chromosome Visualisation Tool (CViT) BLAST search http://medicagohapmap.org/was carried out.本研究对作图杂交群体中7号染色体MtCO区域的DNA标记进行分析，以探究其与spring1位点的连锁关系（详见正文）。包含spring1位点的遗传区间由加粗标注的Medtr7g084170.1与Medtr7g085190.1侧翼界定；每个标记与spring1之间仅发生一次重组事件。 a. 该DNA标记源自明尼苏达大学（University of Minnesota, UMN）蒺藜苜蓿（Medicago truncatula）整合遗传图谱，网址为http://www.medicago.org/genome/map.php。 b. 该DNA标记来自Pierre等人2010年发表的研究。 c. 该PCR扩增片段的大小由http://medicagohapmap.org/提供的Mt3.5基因组组装版本预测得到。 d. 基于“spring1 × Jester”杂交组合的F₂群体，或“测交群体”（详见表1及正文），统计该标记在早花、晚花类群中检测到的重组子数量。此处“测交群体”指（♂(♂spring1 × ♀Jester) × ♀Jester）。NT表示未检测。Indel为插入/缺失（insertion/deletion）；SSR为简单序列重复（Simple Sequence Repeat）。标题为“Mt3.5基因组组装”的列中，给出了经染色体可视化工具（Chromosome Visualisation Tool, CViT）BLAST搜索（搜索网址同为http://medicagohapmap.org）后，各标记在Mt3.5基因组组装版本的拟染色体上的定位信息。