Data from: De novo transcriptome assemblies of four accessions of the metal hyperaccumulator plant Noccaea caerulescens

Noccaea caerulescens of the Brassicaceae family has become the key model plant among the metal hyperaccumulator plants. Populations/accessions of N. caerulescens from geographic locations with different soil metal concentrations differ in their ability to hyperaccumulate and hypertolerate metals. Comparison of transcriptomes in several accessions provides candidates for detailed exploration of the mechanisms of metal accumulation and tolerance and local adaptation. This can have implications in the development of plants for phytoremediation and improved mineral nutrition. Transcriptomes from root and shoot tissues of four N. caerulescens accessions with contrasting Zn, Cd and Ni hyperaccumulation and tolerance traits were sequenced with Illumina Hiseq2000. Transcriptomes were assembled using the Trinity de novo assembler and were annotated and the protein sequences predicted. The comparison against the BUSCO plant early release dataset indicated high-quality assemblies.The predicted protein sequences have been clustered into ortholog groups with closely related species. The data serve as important reference sequences in whole transcriptome studies, in analyses of genetic differences between the accessions and other species, and for primer design.

十字花科（Brassicaceae）天蓝遏蓝菜（Noccaea caerulescens）已成为金属超富集植物（metal hyperaccumulator plants）中的核心模式植物。采自土壤金属浓度各异的不同地理区域的天蓝遏蓝菜种群与种质，其金属超富集与超耐受能力存在显著差异。对多个种质的转录组（transcriptomes）开展比较分析，可为深入解析金属积累、耐受机制及局部适应性提供候选研究对象。该成果可为植物修复专用植物培育及矿物营养改良工作提供重要借鉴。研究人员对4个锌（Zn）、镉（Cd）和镍（Ni）超富集与耐受性状迥异的天蓝遏蓝菜种质的根与茎组织转录组开展了Illumina Hiseq2000测序。转录组组装采用Trinity从头组装软件（Trinity de novo assembler）完成，随后进行了注释与蛋白序列预测。通过与BUSCO植物早期发布数据集（BUSCO plant early release dataset）比对，结果显示本次组装质量优异。预测获得的蛋白序列已与近缘物种的蛋白序列聚类为直系同源基因簇（ortholog groups）。本数据集可作为全转录组研究、种质间及跨物种遗传差异分析以及引物设计的重要参考序列。