Transcriptome-wide mapping of m6A sites with or without YTHDF2 (DF2) knock-down in U2OS cells.

The goal was to see whether and how mRNA stability is linked to m6A peak position in mRNA. The RNA-seq data set in this submission represents a technical RNA-seq repeat of samples 1, 3, 5, 6, 7, and 8 described in a previous GEO submission (GSE181079). Total cytoplasmic mRNA was extracted from U2OS cells with or without DF2 knockd-down. Six RNA samples were collected and fragmented to an average size of about 100nt, which was followed by m6A-RNA IP of m6A site-bearing RNA fragments.本研究旨在探究mRNA稳定性与mRNA上N6-甲基腺苷（m6A）峰位之间是否存在关联及具体作用机制。本次提交的RNA测序（RNA-seq）数据集，是对此前GEO数据库提交项GSE181079中所述的样本1、3、5、6、7、8开展的技术重复RNA测序实验所获得的数据。实验从经DF2基因敲低处理与未处理的U2OS细胞中提取总细胞质mRNA，随后收集6份RNA样本并将其片段化至平均长度约100nt，继而对携带m6A修饰位点的RNA片段实施m6A-RNA免疫沉淀（IP）实验。