Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

Abstract: Rapid tests have been essential to evaluate the physiological potential of seeds and the tetrazolium test is one of those which have been used by seed companies. The objective of the study was to establish the procedure for the tetrazolium test in Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea seeds. For this, three tetrazolium solution concentrations (0.05, 0.075 and 0.1%) and three staining periods (1, 3 and 6 hours) at temperatures of 35 to 40 °C were tested. The seeds were also evaluated by the germination test, whose result was compared with viability by the tetrazolium test. The experimental design was completely randomized in a factorial design 3 x 3 + 1 (three concentrations x three periods + a control = germination test) for each temperature. Viable seeds in the tetrazolium test were compared by Tukey’s test (p ≤ 0.05), while the comparison between the viable seeds with germination test was carried out by Dunnett’s test (p ≤ 0.05). It is recommended that the tetrazolium test for L. ferrea is carried out at a concentration of 0.05% for three-hour staining under 35 °C or 40 °C.摘要：快速检测技术是评估种子生理潜力的核心手段，四唑测定法（tetrazolium test）是种业企业常用的检测方法之一。本研究旨在建立钝叶黄檀（Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea）种子的四唑测定标准化流程。为此，本研究设置3组四唑溶液浓度（0.05%、0.075%及0.1%）与3个染色时长（1、3及6小时），在35℃至40℃的温度条件下开展检测。同时通过发芽试验评估种子活力，并将该结果与四唑测定法得到的种子活力结果进行比对。针对各温度条件，本研究采用3×3+1因子完全随机设计（3个浓度×3个染色时长+1个对照组=发芽试验）。四唑测定法下的活力种子组间差异采用图基检验（Tukey's test，p ≤ 0.05）进行多重比较，而四唑测定法活力结果与发芽试验结果的比对则采用邓尼特检验（Dunnett's test，p ≤ 0.05）完成。本研究建议，钝叶黄檀（L. ferrea）种子的四唑测定可采用0.05%的四唑溶液浓度，在35℃或40℃下染色3小时。