人体皮肤老化的单细胞转录组图谱

皮肤经历不断的自我更新，其功能下降是衰老的明显后果。了解人体皮肤老化需要深入了解各种皮肤细胞类型的分子和功能特性。我们对不同年龄的健康个体的人眼睑皮肤进行了单细胞RNA测序，并鉴定了1种典型细胞类型以及6种基底细胞亚群。进一步的分析显示，随着年龄的增长，与光老化相关的变化逐渐积累，慢性炎症增加。参与发育过程的转录因子在衰老过程中经历了早发性下降。此外，抑制成纤维细胞中的关键转录因子HES1和角质形成细胞中的KLF<>不仅 损害了细胞增殖，而且还增加了衰老过程中的炎症和细胞衰老。最后，我们发现HES<>的基因激活或槲皮素的药物治疗减轻了真皮成纤维细胞的细胞衰老。这些发现为人体皮肤衰老提供了单细胞分子框架，为开发针对衰老相关皮肤病的治疗策略提供了丰富的资源。

The skin undergoes constant self-renewal, and the decline of its functions is a prominent consequence of aging. Understanding human skin aging requires in-depth insights into the molecular and functional properties of diverse skin cell types. We performed single-cell RNA sequencing on human eyelid skin from healthy individuals of different ages, and identified 1 canonical cell type and 6 basal cell subpopulations. Further analysis revealed that age-related changes associated with photoaging gradually accumulate, and chronic inflammation increases with advancing age. Transcription factors involved in developmental processes undergo early-onset decline during aging. Furthermore, inhibiting the key transcription factors HES1 in fibroblasts and KLF<> in keratinocytes not only impaired cell proliferation but also exacerbated inflammation and cellular senescence during the aging process. Finally, we found that genetic activation of HES<> or pharmacological treatment with quercetin alleviated cellular senescence in dermal fibroblasts. Taken together, these findings provide a single-cell molecular framework for human skin aging and serve as a valuable resource for developing therapeutic strategies against aging-related skin diseases.

搜集汇总

背景与挑战

背景概述

该数据集是一个关于人类皮肤老化的单细胞转录组研究，包含72个不同年龄段的眼睑皮肤样本的RNA测序数据。研究揭示了皮肤老化过程中的细胞类型变化、光老化相关特征以及转录因子在衰老中的作用。