Transcriptome analyses of Atlantic salmon muscle genes induced by DNA vaccine against salmon alphavirus subtype 3 (SAV3). Transcriptome analyses of Atlantic salmon muscle genes induced by DNA vaccine against salmon alphavirus subtype 3 (SAV3)

Vaccination of pre-smolt salmon with a plasmid encoding the structural polypeptide of salmonid alphavirus subtype 3 (SAV3) gives protection against infection and development of pancreas disease (PD) mediated with production of antibodies against the virus. The present study analysed transcript responses in the muscle to vaccination with this plasmid (pSAV), pcDNA3.3 was used as a control. pSAV and pcDNA3.3 had similar abilities to up-regulate typical type I IFN stimulated genes. In contrast, pSAV caused higher up-regulation of IFNγ and several IFNγ inducible genes. Compared to pcDNA3.3, pSAV also gave larger increase in transcripts of marker genes for B-cells, T-cells and antigen presenting cells (APCs), which suggests attraction of these cells and that their role in the adaptive immune response elicited by pSAV. Overall design: Fish was recieved intramuscular injection of plasmids or PBS. Muscle samples were collected one week after treatment.

以编码鲑鱼阿尔法病毒3亚型（salmonid alphavirus subtype 3，SAV3）结构多肽的质粒免疫预稚鲑阶段的大西洋鲑，可通过诱导病毒特异性抗体产生，从而抵御该病毒感染及胰腺疾病（pancreas disease，PD）的发生发展。本研究针对该质粒（pSAV）免疫后肌肉组织的转录应答展开分析，以pcDNA3.3作为对照载体。pSAV与pcDNA3.3均能有效上调典型I型干扰素刺激基因的表达，且二者上调能力相近。与之相对，pSAV可更为显著地上调γ干扰素（IFNγ）及其诱导的多种基因的转录水平。相较于pcDNA3.3，pSAV还能更大幅度提升B细胞、T细胞及抗原呈递细胞（antigen presenting cells，APCs）标记基因的转录丰度，这提示pSAV可招募此类免疫细胞，并表明这些细胞在pSAV诱导的适应性免疫应答中发挥了重要作用。实验整体设计：向实验鲑鱼肌肉注射质粒或磷酸盐缓冲液（PBS），于处理后1周采集肌肉组织样本。