Alterations detected in the K22 resistant variants of HCoV-229E.

aDetected by comparison of the nucleotide sequences of HCoV-229E subjected to 10–13 passages in the presence of K22 including its plaque purified variants A-R with those of initial virus or mock-passaged virus (accession number KF293665).bPlaque purified HCoV-229E that served as initial material for the virus passages.cIC50 (µM).dFold resistance to K22 as related to initial virus is shown in parentheses.eVirus preparation and its plaque purified variants M-R obtained in separate K22 selection experiment.fThe virus used for preparation of recombinant nsp6 mutants.gK22 resistant recombinant viruses.a. 通过比对在K22存在条件下经10~13次传代的人类冠状病毒229E（HCoV-229E，含其噬菌斑纯化变体A-R）的核苷酸序列，与原始病毒或模拟传代病毒（登录号：KF293665）的核苷酸序列完成相关检测。b. 用作病毒传代实验起始材料的噬菌斑纯化人类冠状病毒229E（HCoV-229E）。c. 半最大抑制浓度（IC50，单位：µM）。d. 相对于原始病毒的K22耐药倍数标注于括号内。e. 在独立开展的K22筛选实验中获得的病毒制剂及其噬菌斑纯化变体M-R。f. 用于制备重组非结构蛋白6（nsp6）突变体的病毒。g. 对K22产生耐药性的重组病毒。