Cyanobacteria associated with biological soil crusts in Europe. uncultured cyanobacterium

The SCIN (Soil Crust International) project is a multidisciplinary research initiative that aims to achieve improved understanding of the BSCs of Europe, one facet being an investigation into the cyanobacterial communities of BSCs across a latitudinal gradient. Cyanobacteria community assemblages were analysed by both morphological and molecular analysis. Two treatments were applied prior to DNA extraction, continued sample wetting and a dry sample process, 16S ribosomal RNA (rRNA) amplicons were processed by Illumina MiSeq sequencing. The results suggest high and variable cyanobacterial diversity with each site having a unique assemblage.SCIN（Soil Crust International，土壤结皮国际）项目是一项多学科研究计划，旨在深化对欧洲地区生物土壤结皮（Biological Soil Crusts，BSCs）的认知，其研究内容之一是沿纬度梯度对BSCs中的蓝细菌群落展开调查。研究人员通过形态学与分子生物学分析相结合的方式，对蓝细菌群落组成进行了解析。DNA提取前设置了两种预处理方案：持续样品润湿处理与干燥样品处理；随后采用Illumina MiSeq测序平台对16S核糖体RNA（rRNA）扩增子进行了测序分析。研究结果显示，各采样点的蓝细菌多样性水平较高且存在显著变异，每个采样点均拥有独特的群落组成。