Mitotic bookmarking by CTCF and fast post-mitotic gene reactivation in ES cells

Mitosis leads to a global downregulation of transcription that then needs to be efficiently restored. We performed high temporal resolution analysis of the transcriptome as cells exit mitosis, in wild-type mouse ES cells as well as in auxin-depleted Ctcf conditions. RNA-seq quantification of ES cells exiting mitosis有丝分裂（Mitosis）可引发转录的全局性下调，此类下调需被高效恢复。本研究针对细胞退出有丝分裂过程中的转录组开展高时间分辨率分析，实验对象涵盖野生型小鼠胚胎干细胞（ES cells），以及经生长素（auxin）处理以耗竭CCCTC结合因子（Ctcf）的细胞体系，并对退出有丝分裂的胚胎干细胞进行了RNA测序（RNA-seq）定量检测。