Analysis of TC-mAb mice (Exp7: Cell sorting of germinal center B cells)
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http://flowrepository.org/id/FR-FCM-Z5ZU
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The experiment had been performed to isolate germinal center B cells for the analysis of the B cell receptor repertoires of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of germinal center B (CD38lo/-GL7+) in the spleen of unimmunized mice and immunized mice at 14, 21 days after immunization.
Notes:
This experiment corresponds to Supplementary Figure 35 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1
创建时间:
2022-03-01



