longissimus dorsi muscle transcriptome in sheep twins under different feed regimes

To compare the transcriptomes from twin sheep fed with two different feeds, we utilized an Illumina Hi-Seq 2500 to sequence eight longissimus dorsi muscle samples, and generated 22.5 to 28.5 million 125-bp clean paired-end reads. The Trinity software was used to reconstruct a reference transcriptome using obtained RNA-seq reads. This reference transcriptome contained 347335 transcripts with an average length of 2166.29 bp. The expression values of transcripts of the eight samples were calculated by RSEM, whereas the edgeR package in R was used to analyze differentially expressed genes (DEGs) between twin lambs under different diets. Transcriptome analysis of the muscle revealed that the genes related to glucose metabolic processes and fatty acid biosynthesis were significantly differentially expressed between the two groups.为比较饲喂两种不同饲料的双胞胎绵羊的转录组，我们采用Illumina Hi-Seq 2500对8份背最长肌样本进行测序，获得22.5至28.5百万条125 bp的清洁双端测序读段（reads）。本研究利用Trinity软件，基于获取的RNA-seq测序读段重构参考转录组，该参考转录组共包含347335条转录本，平均长度为2166.29 bp。通过RSEM软件计算8份样本中转录本的表达量，并采用R语言中的edgeR软件包，分析不同日粮饲喂的双胞胎羔羊间的差异表达基因（differentially expressed genes，DEGs）。对肌肉组织的转录组分析显示，两组间与葡萄糖代谢过程及脂肪酸生物合成相关的基因存在显著差异表达。