Expression data from fasted macroH2A1/2 double knockout adult mouse liver

MacroH2As core histone variants have a unique structure that includes C-terminal nonhistone domain. MacroH2As are highly conserved in vertebrates, and are thought to regulate gene expression. However the nature of genes regulated by macroH2As and the biological significance of macroH2As for the organism remain unclear. Here we examine macroH2A function in vivo by knocking out both macroH2A1 and macroH2A2 in the mouse. We used microarrays to examine how the absence of macroH2A.1 and macroH2A.2 histone variants affect gene expression fasted adult mouse liver. Two month old male mice were fasted overnight (~16 hours). Mice were sacrificed between 9:00 and 10:00 AM, livers were collected and snap frozen with liquid nitrogen. Total RNA was extract with Trizol (life technologies) following standard protocol.MacroH2A核心组蛋白变体（MacroH2A core histone variants）具有包含C端非组蛋白结构域的独特结构。该蛋白家族在脊椎动物中高度保守，被认为可调控基因表达。然而，MacroH2A所调控的基因类型，以及其对生物体的生物学意义仍未明确。本研究通过敲除小鼠体内的macroH2A1与macroH2A2，在活体水平探究其功能。我们利用基因芯片（microarrays）检测了禁食状态下成年小鼠肝脏中，macroH2A.1与macroH2A.2组蛋白变体缺失对基因表达的影响。实验选取2月龄雄性小鼠，使其禁食过夜（约16小时）；于上午9:00至10:00之间处死小鼠，采集肝脏组织并以液氮快速冷冻。按照标准实验流程，采用Trizol（Life Technologies）提取总RNA。