Tetramorium genome sequencing : combination of Longs/short reads and Hi-C. Tetramorium bicarinatum genome

In a first step library preparation and sequencing were carried out in the Get-PlaGe core facilities with Oxford Nanopore Technologies (ONT, 1D LSK 109) (on GridION instrument) and DNAseq Illumina HiSeq (on HiSeq 3000) and assembled into scaffold on GenoToul bioinformatics facility. Secondly Hi-C library preparation and sequencing (illumina MiniSeq) were performed on MGX-Montpellier GenomiX. These data were used for 3D-DNA scaffolding on the GenoToul bioinformatics facility.

本研究首先在Get-PlaGe核心设施中完成文库制备与测序工作，采用牛津纳米孔科技公司（Oxford Nanopore Technologies, ONT）的1D LSK 109试剂盒（搭载GridION测序仪），以及Illumina公司的DNAseq HiSeq测序方案（搭载HiSeq 3000测序仪），后续于GenoToul生物信息学设施中将所得测序数据组装为支架序列（scaffold）。其次，Hi-C文库的制备与测序（使用Illumina MiniSeq测序仪）在MGX-蒙彼利埃基因组学中心（MGX-Montpellier GenomiX）完成，所得数据被用于GenoToul生物信息学设施中的3D-DNA支架组装流程。