Wheat resistance response triggered upon recognition of the avirulence factor AvrStb6 hinders the penetration of Zymoseptoria tritici through stomata. Wheat resistance response triggered upon recognition of the avirulence factor AvrStb6 hinders the penetration of Zymoseptoria tritici through stomata

Wheat (Chisenese Spring) and Zymoseptoria tritici AvrStb6-expressing virulent and avirulent strains transcriptomes analyses Overall design: We performed a comparative transcriptomic approach to of wheat responses to i) a virulent strain 3D7-GFP, ii) an avirulent strain 3D7-GFP+AvrStb61E4, iii) mock inoculation with water that we used as negative control for infections assays and, iv) the infiltration of AvrStb6 purified protein, v) infiltration of leaves with with PBS buffer used as a negative control for infiltration assay. All treatments were done in triplicates.

中国春小麦（Chinese Spring）与表达AvrStb6无毒基因的小麦壳针孢菌（Zymoseptoria tritici）强毒、无毒菌株的转录组分析
实验设计概述：本研究采用比较转录组学方法，分析小麦对以下五种处理的响应：i) 接种强毒株3D7-GFP；ii) 接种表达AvrStb6无毒基因的无毒株3D7-GFP+AvrStb6^1E4；iii) 以无菌水进行模拟接种，作为侵染实验的阴性对照；iv) 对叶片进行纯化AvrStb6蛋白浸润处理；v) 以PBS缓冲液进行叶片浸润处理，作为蛋白浸润实验的阴性对照。所有处理均设置三次生物学重复。