Analysis of mRNA Expression in Lewis Lung Carcinoma (LLC1) Cells and MLE 12 cells

Whole transcriptome sequencing was used to analyze mRNAs expressed by LLC1 and MLE 12 cells. Overall design: LLC1 or MLE 12 cells were grown in appropriate media to approximately 80% confluence. Cells were isolated and total and mRNA were isolated in order to prepare cDNA libraries.本研究采用全转录组测序（Whole transcriptome sequencing）技术，对LLC1与MLE 12细胞所表达的信使RNA（mRNA）进行分析。实验整体设计：将LLC1或MLE 12细胞置于适宜培养基中培养至细胞汇合度约80%，随后收集细胞并提取总RNA及信使RNA（mRNA），以此制备互补DNA（cDNA）文库。