Figure S1 - CyanoPhyChe: A Database for Physico-Chemical Properties, Structure and Biochemical Pathway Information of Cyanobacterial Proteins
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SDS-PAGE analyses of soluble and insoluble fractions of E. coli expressing the Synechocystis sp. PCC6803 proteins. Solubility of (A) Sll0649, (B) Sll0088 and (C) Sll0359 proteins. 0.4 mM IPTG (final concentration) was added to E. coli cells for inducing expression. The cells were harvested for separation of soluble and insoluble protein fractions, 2 hours after induced expression as described above. IS, Insoluble fraction; S, soluble fraction. Expressed protein bands are shown by open arrows. (DOCX)
表达集胞藻属（Synechocystis sp.）PCC6803蛋白的大肠杆菌（E. coli）可溶性与不可溶性组分的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析。（A）Sll0649、（B）Sll0088与（C）Sll0359蛋白的可溶性分析。以终浓度0.4 mM异丙基-β-D-硫代半乳糖苷（IPTG）诱导大肠杆菌蛋白表达，诱导表达2小时后收集菌体，按前文所述方法分离可溶性与不可溶性蛋白组分。其中IS代表不可溶性组分，S代表可溶性组分；目的蛋白条带以空心箭头标注。 (DOCX)
