Community composition of picoeukaryotes during Noctiluca and Mesodinium bloom Targeted loci environmental. Community composition of picoeukaryotes during successive Noctiluca and Mesodinium bloom

To study the community composition of picoeukaryotes during successive Noctiluca and Mesodinium blooms, 500 mL surface water samples were collected from Port Shelter, Hong Kong, pre-filtered through a 200 μm mesh, and further filtered through a 3.0 µm (47 mm) polycarbonate membrane (PALL Corporation). Then water samples were filtered onto a 0.22 µm (47 mm) polycarbonate membrane for DNA extraction (GE Water and Process Technologies), using a vacuum pump at 0.034 bar Hg or below. The filters were flash frozen and stored at -80ºC until DNA extraction. Samples were then studied using 454-pyrosequencing

为探究夜光藻（Noctiluca）与中缢虫（Mesodinium）相继水华期间的超微型真核生物（picoeukaryotes）群落组成，研究人员从香港吐露港（Port Shelter）采集500 mL表层海水样本。样本先经200 μm孔径网筛预过滤，再依次通过3.0 μm（47 mm）聚碳酸酯滤膜（颇尔公司，PALL Corporation）过滤。随后将水样经0.22 μm（47 mm）聚碳酸酯滤膜（GE水处理及工艺技术公司，GE Water and Process Technologies）截留，采用真空泵维持0.034 bar汞柱及以下的真空度进行抽滤以用于DNA提取。滤膜经快速冷冻后保存于-80℃环境，直至开展DNA提取操作。最终采用454焦磷酸测序技术（454-pyrosequencing）对样本进行分析。