H3-ChIP-Seq and RNA-Seq in A.thaliana SPT16 phospho-mutant lines

FACT consisting of SSRP1 and SPT16 is conserved in a wide variety of plant species. FACT can disassemble and reassemble nucleosomes; thereby it is involved in both overcoming and maintaining the nucleosomal barrier to DNA-dependent processes in the chromatin context. There is only limited information about the potential regulation of FACT function via post-translational modifications. We mutated several identified SPT16 phosphorylation sites followed by RNA-Seq and H3-ChIP-Seq as a measurement for total genome-wide nucleosome occupany in the mutant lines. The expression of a non-phosphorylatable SPT16 variant results in a distinct rearrangement of H3.