The mRNA expression profiles of the hypocotyl and root in WT and hy5. The mRNA expression profiles of the hypocotyl and root in WT and hy5

The mRNA expression profiles of the hypocotyl and root in WT and hy5 were analyzed in 24 hour 22degC and 28degC treated condition. The gene expression changes were analyzed and compared within 22degC and 28degC and in different tissue type (hypocotyl vs root). Overall design: Total twentiefour 24h 22degC and 28degC treated Arabidopsis samples were used. These include three replicates of following genotypes; Col-0 and hy5, with two tissue type; hypocotyl and root. Therefore, three replicates of following genotypes, tissue and conditions; Col-0 (hypocotyl-22degC), Col-0 (root-22degC), hy5 (hypocotyl-22degC), hy5 (root-22degC), Col-0 (hypocotyl-28degC), Col-0 (root-28degC), hy5 (hypocotyl-28degC), hy5 (root-28degC).

本研究分析了野生型（Wild Type, WT）与hy5突变体在下胚轴（hypocotyl）和根（root）组织中的信使RNA（messenger RNA, mRNA）表达谱，实验设置22℃与28℃两种温度处理条件，处理时长均为24小时。随后对基因表达变化进行分析，并在22℃与28℃处理条件下，以及不同组织类型（下胚轴vs根）间开展比较分析。整体实验设计：共使用24份经24小时22℃与28℃处理的拟南芥样本。实验包含两种基因型（Col-0与hy5）各3次生物学重复，以及两种组织类型（下胚轴与根）。具体而言，各处理组合均设置3次生物学重复，具体组别如下：Col-0（下胚轴-22℃）、Col-0（根-22℃）、hy5（下胚轴-22℃）、hy5（根-22℃）、Col-0（下胚轴-28℃）、Col-0（根-28℃）、hy5（下胚轴-28℃）、hy5（根-28℃）。