Human 32 MPSS samples

This series represents a very deep survey of all transcripts expressed in a wide range of human tissues and cells by Massively Parallel Signature Sequencing (MPSS). These datasets have been generated and donated to the scientific community by Lynx Therapeutics, Inc in Hayward, CA. The data contained in this submission is described in the following publication: 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Abstract of the publication: 'We have used massively parallel signature sequencing (MPSS) to sample the transcriptomes of 32 normal human tissues to an unprecedented depth, thus documenting the patterns of expression of over 20,000 genes with high sensitivity and specificity. The data confirm the widely held belief that differences between cell and tissue types are largely determined by the expression of a limited number of tissue-specific genes, rather than by combinations of more promiscuously expressed genes. Expression of a little over half of all known human genes seems to account for both the common requirements and the specific functions of the tissues sampled. A classification of tissues based on patterns of gene expression largely reproduces classifications based on anatomical and biochemical properties. The unbiased sampling of the human transcriptome achieved by MPSS supports the notion that most human genes have been mapped, if not functionally characterised. This dataset should prove useful for the identification of tissue-specific genes, for the study of global changes induced by pathological conditions, and for the definition of a minimal set of genes necessary for basic cell maintenance.' Keywords: other

本系列数据集是借助大规模平行信号测序（Massively Parallel Signature Sequencing, MPSS），针对覆盖广泛人类组织与细胞的全部表达转录本所开展的深度全景调查。该数据集由位于加利福尼亚州海沃德市的Lynx Therapeutics, Inc公司生成并捐赠给科学界。本次提交所包含的数据已在以下出版物中详细说明：《C. Victor Jongeneel、Mauro Delorenzi、Christian Iseli、周代兴（Daixing Zhou）、Christian D. Haudenschild、Brian J. Stevenson、Robert L. Strausberg、Andrew J.G. Simpson 及 Thomas J. Vasicek. 基于大规模平行信号测序（MPSS）的人类基因表达图谱. 基因组研究（Genome Research）VolXX, 2004》。该出版物的摘要如下："我们借助大规模平行信号测序（MPSS），对32种正常人类组织的转录组进行了前所未有的深度采样，以高灵敏度与特异性记录了超过20000个基因的表达模式。本数据证实了学界广泛认可的观点：细胞与组织类型间的差异，主要由有限数量的组织特异性基因的表达所决定，而非由大量广谱表达基因的组合所主导。仅约半数已知人类基因的表达，即可同时满足所采样组织的基本生存需求与特定功能。基于基因表达模式的组织分类，在很大程度上重现了基于解剖学与生化特性的分类结果。通过MPSS实现的人类转录组无偏采样，支持了‘即便尚未完成功能注释，绝大多数人类基因也已完成定位映射’这一学术观点。本数据集可用于组织特异性基因的筛选、病理状态诱导的全局表达变化研究，以及定义维持细胞基本存活所需的最小基因集。"关键词：其他