Systematic Engineering of Artificial Metalloenzymes for New-to-Nature Reactions

Data underlying the figures in the publication “Systematic Engineering of Artificial Metalloenzymes for New-to-Nature Reactions”, published in Sci. Adv., <strong>2021</strong>, 7, eabe420<em>RSC. </em> <em>https://advances.sciencemag.org/content/7/4/eabe4208.abstract</em> Table of contents: <strong>1. Dataset 1</strong>; Excel file containing the dataset for the Figures in the publication. It contains the activity of 400 artificial metalloenzymes measured for five reactions. In addition, the expression level of all mutants as well as validation experiments in vivo and in vitro are provided. As outlined in the file, some data were processed by subtracting a blank and normalizing to the optical density of the cultures as well as to a wild type control. Mutants are referred to by the amino acids at positions 112 and 121 of the protein.本数据集为发表于《科学·进展》（*Science Advances*，缩写Sci. Adv.）2021年第7卷、论文编号eabe4208、题为《用于全新天然反应的人工金属酶系统工程》（Systematic Engineering of Artificial Metalloenzymes for New-to-Nature Reactions）的配图支撑数据，论文链接：https://advances.sciencemag.org/content/7/4/eabe4208.abstract。 数据集目录：1. 数据集1；该Excel文件包含本论文配图所用的全部数据集，收录了针对5种反应测得的400种人工金属酶（artificial metalloenzymes）的活性数据。此外，还提供了所有突变体（mutants）的表达水平数据，以及体内（in vivo）和体外（in vitro）验证实验数据。 如文件所述，部分数据已完成预处理：扣除空白对照，并以培养物的光密度（optical density）以及野生型对照为基准进行归一化处理。突变体以该蛋白质第112位和第121位的氨基酸残基进行命名标识。