Dendrobium nobile , Dendrobium wardianum and their F1 progeny Raw sequence reads
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100 F1 plants from a interspecific hybridization between Dendrobium nobile and D. wardianum was constructed based on the pseudo-testcross strategy. RNA sequencing (RNA-seq) approach was employed to identify a large number of SNP markers for parents and progeny,and 9645 SNP markers were organized into a high-density genetic map.
本研究基于拟测交(pseudo-testcross)策略,构建得到金钗石斛(Dendrobium nobile)与翅梗石斛(Dendrobium wardianum,原文缩写为D. wardianum)种间杂交的100株F1代植株。采用RNA测序(RNA-seq)技术对亲本及该群体进行测序,鉴定得到大量单核苷酸多态性(Single Nucleotide Polymorphism,SNP)标记,最终将9645个SNP标记整合构建为一张高密度遗传连锁图谱。
创建时间:
2018-05-10



