Quantitative DIA-based proteomic analysis of lysosomes (Lyso-IP) from VPS35[D620N] Mouse Brain.

This Zenodo deposit contains a publicly available description of the Dataset: Title: "Quantitative DIA-based proteomic analysis of lysosomes (Lyso-IP) from VPS35[D620N] Mouse Brain.". Description: Proteomic characterization of brain lysosomes comparing WT and VPS35[D620N] mutant mice. Tissue was processed via glass-Teflon homogenization and 3-minute Lyso-IP. Data acquired on an Orbitrap Exploris 480 and processed using DIA-NN 1.8.1. This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset本Zenodo存档包含该数据集的公开说明： 标题："源自VPS35[D620N]小鼠大脑的溶酶体基于数据非依赖采集（Data-Independent Acquisition，DIA）的定量蛋白质组学分析（Lyso-IP）" 数据集说明：本研究针对野生型（Wild Type，WT）与VPS35[D620N]突变小鼠的大脑溶酶体开展蛋白质组学表征。实验组织样本采用玻璃-特氟龙匀浆法处理，并进行3分钟的Lyso-IP操作；质谱数据通过Orbitrap Exploris 480采集，随后使用DIA-NN 1.8.1软件完成数据分析。 本数据集通过ASAP CRN云平台（cloud.parkinsonsroadmap.org）向科研人员开放；访问申请流程可参阅用户手册。 本研究由帕金森病研究协同网络（Aligning Science Across Parkinson's Collaborative Research Network，ASAP CRN）通过迈克尔·J·福克斯帕金森病研究基金会（Michael J. Fox Foundation for Parkinson's Research，MJFF）资助。 本Zenodo存档由ASAP CRN云平台工作人员代表数据集作者创建，可为CRN云平台数据集提供可引用的学术参考文献。