Structure of transmembrane domain of mouse NBCe1 by homology modeling

The structure model of the transmembrane domain of mouse NBCe1 was generated by SWISS-MODEL with the cryo-EM structure of human NBCe1 (PDB ID: 6CAA) as the template. Residues 445–1007 of mouse NBCe1-B was aligned with the homologous region of human NBCe1 by using SWISS-MODEL. The sequence identity between the two sequences is 96.09%. Structural assessment shows that the simulated model of mouse NBCe1 had QMEAN value −6.86, Cβ −3.57, solvation value −1.80, torsion value −5.49, and scored 1.49 by MolProbity approach.以人源NBCe1的冷冻电镜（cryo-electron microscopy, cryo-EM）结构（蛋白质数据库[Protein Data Bank, PDB]编号：6CAA）为模板，通过SWISS-MODEL构建了小鼠NBCe1跨膜结构域的结构模型。通过SWISS-MODEL将小鼠NBCe1-B的第445至1007位残基与人源NBCe1的同源区域进行了序列比对，二者的序列一致性为96.09%。结构评估结果显示，该小鼠NBCe1的模拟模型的QMEAN值为-6.86、Cβ值为-3.57、溶剂化值为-1.80、扭转角值为-5.49，经MolProbity方法评估得分为1.49。