Increased plankton diversity and community similarity in artificially heated lakes

We collected lake water samples from a set of five heated lakes (Gosławskie, Licheńskie, Pątnowskie, Ślesińskie and Mikorzyńskie) and five unheated (hereafter “control”) lakes (Budzislawskie, Gopło, Skulskie, Skulska Wieś and Suszewskie) located near the city of Konin in central Poland. Each lake was sampled four times: in winter, spring, summer and autumn 2020. Two liters of surface water were collected at each sampling event and passed through a glass fiber filter (Whatmann GF/F, 25 mm diameter, 0.7 μm pore size) using vacuum filtration at 200 mbar. DNA was extracted from the whole pulverized filter using the NucleoSpin Plant II extraction kit (Machery-Nagel, Düren, Germany) following the manufacturer’s protocol. All samples were amplified using three primer sets (hereafter, DIV4, EUK15 and CHY) that target a small region of either the 18S (DIV4 and EUK15) or 28S (CHY) rRNA genes of eukaryotes. Amplicons were then pooled in equimolar amounts and sequenced on an Illumina MiSeq using a v3 sequencing kit (600 cycles). The data offers a great opportunity to address potential long-term effects of warming on freshwater communities across seasons and trophic levels.

我们于波兰中部科宁（Konin）市周边采集了湖水样本，涵盖5座加热型湖泊（heated lakes，Gosławskie、Licheńskie、Pątnowskie、Ślesińskie及Mikorzyńskie湖）与5座未加热的对照（control）湖泊（Budzislawskie、Gopło、Skulskie、Skulska Wieś与Suszewskie湖）。所有湖泊均于2020年冬、春、夏、秋四季各开展1次采样：每次采样采集2升表层湖水，以200 mbar负压过滤法，通过Whatmann GF/F型玻璃纤维滤膜（直径25 mm，孔径0.7 μm）完成过滤。随后采用NucleoSpin Plant II提取试剂盒（Machery-Nagel，德国迪伦），按照制造商提供的操作流程，从经研磨的整块滤膜中提取脱氧核糖核酸（DNA）。所有样本均使用3对引物组进行扩增，下文分别记为DIV4、EUK15与CHY，这3对引物分别靶向真核生物18S（DIV4与EUK15）或28S（CHY）核糖体RNA（rRNA）基因的一段小区域。将所得扩增子（amplicons）以等摩尔浓度混合后，使用Illumina MiSeq平台搭配v3测序试剂盒（600个循环）完成测序。本数据集为探究气候变暖对淡水群落跨季节及营养级的潜在长期影响提供了绝佳的研究契机。