Free fatty acids and TNF-alpha in hepg2/c3a cell culture

free fatty acids(palmitate, oleate, linoleate) 0.7mM and tnf-alpha (0 20,100 ng/ml) were subjected to HepG2 cell line to study the cytotoxicity induced by these two factors Keywords: stress response control(Hepg2 medium and BSA medium) treatment(combinations of TNFa+FFAs) two biological replicates for each condition, color swap for each sample本实验将浓度为0.7mM的游离脂肪酸（free fatty acids，涵盖棕榈酸酯、油酸酯、亚油酸酯）与浓度梯度为0、20、100 ng/ml的肿瘤坏死因子α（TNF-α）处理HepG2细胞系（HepG2 cell line），以探究这两种因子诱导的细胞毒性。关键词：应激反应对照（stress response control，采用HepG2培养基与牛血清白蛋白培养基）、处理组（treatment，即肿瘤坏死因子α与游离脂肪酸的联合处理）、每个实验条件设置2次生物学重复，且所有样本均进行颜色互换（color swap）。