RNA profiling from ovarian FFPE specimens (Nanostring). Homo sapiens

To study feasibility of gene expression profiling from FFPE tissues using NanoString nCounter platform, we designed a pilot study utilizing samples from ovarian cancer cohort. We selected samples from large-scale epidemiologic studies and clinical trials representative of a wide variety of fixation times, block ages and block storage conditions. Overall design: five serous carcinoma and six clear cell carcinoma samples with technical replicates为探究利用NanoString nCounter平台开展福尔马林固定石蜡包埋（Formalin-Fixed Paraffin-Embedded, FFPE）组织的基因表达谱分析的可行性，本研究设计了一项基于卵巢癌队列样本的预试验。本研究选取的样本来自大规模流行病学研究与临床试验，覆盖了多样的组织固定时长、石蜡块存放时长及石蜡块储存条件。试验整体设计：共纳入5例卵巢浆液性癌（serous carcinoma）样本与6例卵巢透明细胞癌（clear cell carcinoma）样本，所有样本均设置了技术重复（technical replicates）。