Genome-wide transcriptome sequencing at different stages of Drosophila development, RNA-seq

This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains total RNA sequencing data generated on Solexa Genome Analyzer for whole Drosophila animals at 12 different time-points of development. Keywords: Epigenetics For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf For each time-point (E-0-4h, E-4-8h, E-8-12h, E-12-16h, E-16-20h, E20-24h, L1, L2, L3, Pupae, Adult Males and Adult Females) a total RNA extraction has been performed. After conversion into double stranded DNA, the samples have been sequenced in duplicate on Solexa Genome Analyzer following Solexa sequencing procedure.本数据集由芝加哥大学Kevin P. White教授领衔的果蝇调控元件modENCODE项目生成。该数据集包含针对果蝇完整个体12个不同发育时间节点的总RNA测序数据，测序平台为Solexa基因组分析仪。关键词：表观遗传学（Epigenetics）。有关数据使用条款与条件，请参阅：http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。针对以下12个发育时间节点（胚胎期0-4小时（E-0-4h）、胚胎期4-8小时（E-4-8h）、胚胎期8-12小时（E-8-12h）、胚胎期12-16小时（E-12-16h）、胚胎期16-20小时（E-16-20h）、胚胎期20-24小时（E20-24h）、第一龄幼虫（L1）、第二龄幼虫（L2）、第三龄幼虫（L3）、蛹（Pupae）、成年雄果蝇（Adult Males）与成年雌果蝇（Adult Females）），均已完成总RNA提取操作。样本经双链DNA转化后，按照Solexa测序流程在Solexa基因组分析仪上进行了两次重复测序。