Transcriptomics of CD29+/CD44+ cells isolated from hPSC retinal organoids reveals a cell population with retinal progenitor and M?ller glia characteristics.

M?ller glia play very important and diverse roles in retinal homeostasis and disease, bur very little is known of their development during human retinal embryogenesis. Since they share several markers with retinal progenitors, they are often considered as a different cell population. In this study we isolated CD29+/CD44+cells from retinal organoids formed by hEPSC cells in vitro, and examined their transcriptome profile at various stages of organoid development to identify their transcriptomic profile.

米勒胶质细胞（Müller glia）在视网膜稳态与疾病进程中发挥着极为重要且多样的作用，但目前对于人类视网膜胚胎发生过程中其发育机制的认知仍十分有限。由于该类细胞与视网膜祖细胞共享多种标志物，因此常被视为一类独立的细胞群。本研究从体外由人类扩增多能干细胞（hEPSC）构建的视网膜类器官中分离出CD29+/CD44+细胞，并在类器官发育的不同阶段对其转录组特征进行检测，以明确该细胞群的转录组表达谱。