Growth, sporulation, dry weight, melanin, chitin and ergosterol data for Rhizopus microsporus at 30 °C and 37 °C
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This record contains two Excel workbooks with raw measurements and post-hoc statistics for Rhizopus microsporus strains UM6 (wild type) and UM120.2/UM120.5 (choC mutants). Data provides: Phenotypic assays (growth, sporulation, biomass): Sheets: “30 °C Growth” and “37 °C Growth” (colony diameter in cm, Days 1–5); “30 °C Sporulation” and “37 °C Sporulation” (spores/cm³, Days 3 and 5); and “30 °C Dry Weight” (mycelial dry weight in mg, Days 1–7; measured at 30 °C only). Three replicate measurements are provided per strain and time point. Companion “Tukey’s test …” sheets report mean differences, 95% CIs, significance, and adjusted P values. Cell-wall & membrane components (melanin, chitin, and ergosterol): Sheets: “Melanin Quantification” (absorbance at 405 nm; n=3 per strain), “Chitin Quantification” (Mean Staining Index; n=3 per strain), and “Ergosterol Quantification” (ergosterol content, %; n=4 per strain). Companion “Tukey’s test ...” sheets provide the corresponding multiple-comparisons statistics. These processed data analyses reported in the manuscript “Phosphatidylcholine Biosynthesis via ChoC is Crucial for Cellular Integrity and Virulence in Rhizopus microsporus”.
本数据集包含两份Excel工作簿,内含米根霉(Rhizopus microsporus)菌株UM6(野生型)与UM120.2/UM120.5(choC突变体)的原始测量数据及事后统计分析结果。 数据集包含以下内容: 1. 表型检测(生长、产孢、生物量):对应工作表"30 °C 生长"与"37 °C 生长"(记录第1至5天的菌落直径,单位:cm);"30 °C 产孢"与"37 °C 产孢"(记录第3、5天的孢子密度,单位:孢子/cm³);以及"30 °C 干重"(仅在30 °C下测定,记录第1至7天的菌丝干重,单位:mg)。每个菌株在每个时间点均设置3次重复测量。配套的"Tukey检验(Tukey's test)"工作表提供均值差异、95%置信区间(CI)、显著性水平与校正后P值。 2. 细胞壁与膜组分(黑色素、几丁质与麦角固醇):对应工作表"黑色素定量"(记录405 nm波长处的吸光度,每个菌株n=3)、"几丁质定量"(记录平均染色指数,每个菌株n=3)与"麦角固醇定量"(记录麦角固醇含量,单位:%,每个菌株n=4)。配套的"Tukey检验(Tukey's test)"工作表提供对应的多重比较统计结果。 本数据集的处理分析结果已刊载于论文《Phosphatidylcholine Biosynthesis via ChoC is Crucial for Cellular Integrity and Virulence in Rhizopus microsporus》。



