Optimization of protease production by the fungusMonacrosporium thaumasium and its action againstAngiostrongylus vasorum larvae

The objectives of this study were to optimize protease production from the nematophagous fungus Monacrosporium thaumasium (NF34a) and evaluate its larvicidal activity and biological stability. An isolate of the nematophagous fungus Monacrosporium thaumasium (NF34a) was used to produce the enzyme. The Plackett-Burman design was used in order to scan which components of the culture medium could have a significant influence on protease production by the fungus NF34a. An in vitro assay was also performed to evaluate the larvicidal activity of NF34a. It was observed that only one component of the culture medium (yeast extract), at the levels studied, had any significant effect (p本研究旨在优化食线虫真菌单顶孢霉（Monacrosporium thaumasium）NF34a的蛋白酶生产工艺，并评价其杀幼虫活性与生物学稳定性。本研究采用食线虫真菌单顶孢霉（Monacrosporium thaumasium）NF34a菌株制备该蛋白酶。为筛选对NF34a菌株蛋白酶生产具有显著影响的培养基组分，本研究采用普拉凯特-伯曼（Plackett-Burman）设计。此外，本研究还开展了体外实验以评价NF34a菌株的杀幼虫活性。研究观察到，在所考察的浓度水平下，培养基中仅酵母提取物这一组分对蛋白酶生产具有显著影响（p