An Evaluation of Sequence Capture Technologies with Polyploid Cotton

Sequence capture is a revolutionary method to resequence targeted portions of the genome but it has not been tested with a polyploid plant. We constructed two capture platforms 1) a novel Nimblegen sequence capture microarray and 2) Mycroarray beads containing RNA probes. Both platforms targeted the same 534 genes. On each platform, we hybridized two different accessions to this microarray to assess capture efficiency and potential of combined sequence using DNA multiplex identifiers (MIDs). For more information, visit http://www.gossypium.info序列捕获（sequence capture）是一种可对基因组靶标区域进行重测序的革命性技术，但目前尚未在多倍体植物中开展相关测试。本研究构建了两款捕获平台：1）新型Nimblegen序列捕获微阵列；2）搭载RNA探针的Mycroarray磁珠。两款平台均靶向同一组共534个基因。针对每款平台，我们将两份不同的植物种质与该微阵列进行分子杂交，以利用DNA多重标识符（MIDs）评估捕获效率与复合序列测序潜力。如需获取更多信息，请访问 http://www.gossypium.info